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Evaluation of essential oil composition of dudiam melon and antimicrobial activity of essential oil and extract of that
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Manijeh Chakaneh   , Akram Sharifi *  , Armineh Roshanpazhoh , Alireza Motavalizadeh Kakhky |
| Department of Food Science and Technology, Qazvin Branch, Islamic Azad University, Qazvin, Iran |
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Abstract: |
Dudiam melon has a high nutritional value. The bioactive compounds found in the essential oil and extract of this fruit can be used to control the growth of pathogenic bacteria of food origin or spoilage bacteria. In this study, the essential oil of Cucumis melo var. dudaim fruit was extracted by steam distillation method. Methanolic, n-hexane, chloroform, ethyl acetate extracts were also produced by maceration method. The essential oil compounds of this fruit were identified by gas chromatograph (GC) and gas chromatograph connected to mass spectrometer (GC-MS) devices, and the percentage of its compounds was measured. In order to determine the minimum inhibitory concentration of the obtained essential oil and extracts on Escherichia coli, Streptococcus pneumonia, Staphylococcus aureus and Pseudomonas aeruginosa, the Disk Diffusion method was used. The results showed in fruit essential oil, Dehydro Aromadendrene, (Z)-Calamenene and (Z)-Nerolidol are the major compounds, respectively, and the results obtained from the microbial tests showed that the studied bacteria have a significant difference in level of 5% in terms of diameter of non-growth halo. The lowest non-growth diameter halo was related to Escherichia coli bacteria and the highest non-growth diameter halo was related to Pseudomonas aeruginosa bacteria. The minimum inhibitory concentration was for Pseudomonas aeruginosa bacteria. Overall, the fruit essential oil showed more antimicrobial activity against the bacteria than its extracts. These herbal compounds can be used as substitutes for chemical preservatives in foods. |
Article number: 6 |
| Keywords: Dudiam Melon, Essential Oil, Extract, Minimum Inhibitory Concentration. |
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Type of Study: Research |
Subject:
General
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