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Optimization of media components for biomass production of the probiotic strain Lactobacillus delbrueckii subsp. bulgaricus using experimental design
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Morteza Mohajeri Amiri *   , Seyyedeh Masumeh Mirnurollahi |
| Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran. |
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Abstract: |
The Lactobacillus delbrueckii subsp. bulgaricus is one of the most well-known probiotic lactic acid bacteria, which is traditionally used in fermented products, especially dairy products. Few studies have focused on the optimization of L. delbrueckii subsp. bulgaricus biomass production. Therefore, it is necessary to use the design of the experiment and response surface methodology in the optimization of this important probiotic strain. The L. delbrueckii subsp. bulgaricus strain isolated from fermented products of Tehran province was used in this study. By studying one-factor-at-a-time, carbon and nitrogen sources were evaluated. Three factors of glucose, yeast extract and peptone were confirmed to be evaluated in the optimization test. Then, using the experimental design and response surface methodology, the Box-Behnken model was used to optimize these three variables. The results obtained from Box-Behnken design with statistical software identified the full quadratic model for significant optimization. This model suggests that in the concentration of glucose 20 gr/L and the concentration of yeast extract 25 gr/L and peptone 30 gr/L, a dry weight of 2.132 gr/L is obtained, which was confirmed. This study has been successful in optimizing carbon and nitrogen sources on L. delbrueckii subsp. bulgaricus biomass production. The variables and values obtained are confirmed by other studies, but finally, due to the restricted studies that have been done on the optimization of the cultivation conditions of this probiotic strain, more investigations are suggested to confirm the findings of this study.
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| Keywords: Lactobacillus delbrueckii subsp. bulgaricus, one-factor-at-a-time, Box-Behnken model, biomass, Response Surface Methodology. |
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Type of Study: Research |
Subject:
General
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