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Detection of bovine tuberculosis using indirect ELISA method of low molecular weight antigen of Mycobacterium bovis strain AN5
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Malihe Honarmand Kashi    , Nader Mosavari , Mitra Salehi * , Naheed Mojgani |
| Department of Biology, Islamic Azad University of North Tehran Branch, Tehran, Iran. |
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Abstract: |
The determination of bovine tuberculosis is made with different tests and with certain limitations. Antigenic proteins can be extracted from Mycobacterium bovis culture that can improve the sensitivity and specificity of diagnostic tests. One of the targets of this study is to design an indirect ELISA. For this reason, purified Mycobacterium bovis AN5 antigen were utilized to distinguish bovine tuberculosis. Another goal is to review and compare the results of the system designed to Interferon-ɣ (IFN-ɣ) assay. Mycobacterium bovis AN5 antigens were isolated and purified. The selected antigen was then used to design the indirect ELISA system. The sera of 230 cows from different farms were tested with designed ELISA. Their results were at that point compared with interferon-gamma test as a corroborative diagnostic test. Correlation and independent study between variables were performed by Pearson and T test. The sensitivity and specificity of the designed indirect ELISA system were 77.8% and 80.7%, respectively. The p value for Pearson and T test was zero percent. Noteworthy lightweight antigens were purified that might analyze the disease. Due to the high sensitivity and specificity gotten, the ELISA system outlined for starting screening within the diagnosis of bovine tuberculosis. |
Article number: 6 |
| Keywords: Key words: Mycobacterium bovis, antigenic protein, gamma interferon, indirect ELISA. |
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Full-Text [PDF 1350 kb]
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Type of Study: Research |
Subject:
Special
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