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Phenotypic and Genotypic Analysis of the Prevalence of TEM, SHV, and CTX-M Extended-Spectrum Beta-Lactamase Genes in Escherichia. coli Strains Isolated from Clinical Samples of Hospitalized Patients in Tehran Using PCR
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پدرام ح حیدری *   , Raziyeh Taraghlizadeh Marzroud |
| - Department of Microbiology, Khoy Branch, Islamic Azad University, Khoy, Iran. |
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Abstract: |
In recent years, the increase in Escherichia coli strains producing extended-spectrum beta-lactamases (ESBLs) has limited treatment options. This study was conducted on 100 E. coli isolates over a period of 3 months. The sensitivity of the isolated bacteria to 7 types of antibiotics was determined using the disk diffusion method according to the CLSI 2021 standard guidelines. The strains were examined for the presence of ESBL enzymes using the combined disk test (CDDT). The CTX-M, SHV, and TEM genes were identified using PCR, and some isolates were sequenced using the Sanger method and blasted in the NCBI database. Among the 42 isolated E. coli strains, resistance to ampicillin, ceftazidime, ciprofloxacin, imipenem, nalidixic acid, ceftriaxone, and gentamicin was 90.5%, 85.7%, 59.5%, 52.4%, 40.5%, 35.7%, and 9.5%, respectively. Out of 23 isolates (54.8%) that were ESBL positive, 78.3% carried the CTX-M gene, 73.9% harbored the TEM gene, and 21.7% had the SHV gene.The prevalence of ESBL-producing E. coli strains in this study indicates that ESBL production and its prevalence vary from city to city. Therefore, the use of antibiogram methods, molecular diagnosis, and identification of different types of extended-spectrum beta-lactamase genes should be periodically performed in all healthcare centers and hospitals.
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| Keywords: Escherichia coli, Extended-Spectrum Beta-Lactamase, Disk diffusion, ESB |
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Type of Study: Research |
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