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Investigating the expression of thrB and thrC genes of Corynebacterium glutamicum ATCC13032 in the laboratory production of threonine
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Nadiya Abbasi yousefabad   , Nastaran Daryai Ghazani , Leila Rehbarnia * , Alireza Dehnad |
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Abstract: |
threonine amino acid is one of the essential amino acids for livestock and poultry, therefore its use as a food supplement is of particular importance in nutrition. Today, one of the common methods of threonine production is the use of microorganisms, especially Corynebacterium glutamicum. Fast production, easy cultivation and low production cost are the advantages of using this bacterium to produce threonine amino acid. In this study, c.glutamicum (ATCC13032) was cultured in the standard culture medium called ISP-2, and then to optimize the ISP-2 culture medium, the effect of additives such as cheese water, bagasse, molasses, and corn syrup on threonine production tested Threonine production rate was evaluated using chromatography method. After reviewing the results, the best physical and chemical conditions for threonine production were selected and the expression levels of key genes involved in threonine production, including thrB and thrC, were analyzed by Real-time PCR technique. The results obtained from chromatography showed that the concentration of 0.4 grams per liter of cheese water provides the best conditions for the production of threonine. So that the concentration of threonine produced increased by 33271.53 mg/L in the cheese water sample. Based on Real-time PCR results, cheese water culture medium increased thrB gene expression by 2 times and thrC expression by 1.08 times. Based on the obtained results, physicochemical changes in the culture medium can have a significant effect on threonine production by Corynebacterium glutamicum bacteria.
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| Keywords: threonine, Corynebacterium glutamicum, thrB and thrC gene expression |
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Type of Study: Research |
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