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Optimization of the culture of Phaffia rhodozyma yeast to obtain maximum biomass
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Maryam Ameri   , Reza Karazhyan * , Najem Gord- noshahri , Pair rokh Lavaee , Omid Safari , Mohsen Seyedabadi |
| Research Institute for Industrial Biotechnology, Department of Industrial Microbial Biotechnology, ACECR, Mashhad, Iran |
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Abstract: |
Astaxanthin is a powerful pigment and antioxidant used in the food, aquaculture, cosmetics, and pharmaceutical industries. Its market is expected to reach $4.8 billion by 2028. Phaffia rhodozyma with astaxanthin synthesis and weak wall is a suitable option for commercial pigment production. In this study, the yeast Phaffia rhodozyma was used. After the strain was activated, the yeast cells were grown in different broth cultures with different amounts of carbon and nitrogen sources and each culture medium was placed at three different pHs (5.5, 6, and 6.5) and at two different temperatures (15°C and 21°C) with the same stirring speed (100 rpm). Growth in each of the environments under different conditions was measured with a spectrophotometer on consecutive days for up to 14 days, and different models were applied to determine maximum growth and other factors. The optimal cultivation conditions were also determined. The optimal cultivation conditions for Phaffia rhodozyma yeast for the main culture medium 3 are between 17°C and 19°C, which is the appropriate and optimal temperature for cultivation, and the suitability of the conditions has increased with increasing pH from 5.5 to 6.5.
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| Keywords: Phaffia rhodozyma, cultivation, optimization |
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Type of Study: Research |
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