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The frequency of Vibrio parahaemolyticus isolated from Caspian Sea fishes and their antibacterial resistance pattern
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Elaheh Yazdanpanah   , Zahra Rajabi , Mohammad Kazem Sharifi Yazdi , Msoltandallal Msoltandallal * |
| 1-Division of Food Microbiology, Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.2- Food Microbiology Research Centre, Tehran University of Medical Sciences, Tehran, Iran |
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Abstract: |
| Vibrio parahmolyticus is a marine and estuarine bacterium that damages the aquatic industry due to food-related outbreaks and is a great threat to food safety and human health. Annually, many reports of food poisoning occur due to consumption. Seafood contaminated with Vibrio bacteria is released. The present study was conducted in order to investigate the frequency of Vibrio parahaemolyticus isolated from Caspian sea fish and to determine the pattern of antibacterial resistance.For five months (June 2021 to October 2021), 220 samples of fish caught from the Caspian Sea were prepared from the Gilan Fisheries Department with full compliance with health conditions. To isolate Vibrio parahemolyticus, after enrichment in buffered peptone water for 24 hours at 37°C, a loop was cultured in thiosulfate citrate bile sucrose agar medium for 24 hours at 37°C. The day after the colony yellow and green suspects are used from standard differential tests. In addition, to determine the pattern of antibiotic resistance according to CLSI guidelines, antibiotic disks of MAST Company were used. Out of a total of 220 fish samples caught from the Caspian Sea, 759% of the fish samples were infected with Vibrio species. Vibrio parahaemolyticus abundance was reported as 19%, which was more than the other species obtained.Also, the results obtained from the antibiotic resistance pattern indicate the sensitivity of this strain to ciprofloxacin antibiotics (98.9%, gentamycin (87.8%), chloramphenicol (92.4%) and their resistance to amoxicillin (96.3%), doxycyclin (94.1%) and tetracycline (92.3%). Our findings showed that the fish studied in the Caspian Sea are infected with Vibrio parahaemolyticus, which can be considered a serious threat to the health of seafood consumers. |
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| Keywords: Vibrio parahaemolyticus, Fish, Caspian Sea, Antibiotic Resistance |
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Type of Study: Research |
Subject:
General
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