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Evaluation of the cytotoxic, antioxidant, and antitumor effects of Exopolysaccharide extracted from Lactobacillus plantarum isolated from traditional dairy products of Guilan province
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Masoumeh Anvari *   , Farshid Farajollahi Nia , Khosro Isazadieh |
| Department of Biology Ra.C., Islamic Azad University, Rasht |
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Abstract: |
Exopolysaccharides derived from lactic acid bacteria have proven beneficial and nutritional properties. Therefore, this study aimed to evaluate the anticancer, antioxidant, and antitumor effects of polysaccharides extracted from lactic acid bacteria isolated from traditional dairy products in Guilan province in order to select exopolysaccharide-producing strains for therapeutic and industrial purposes. In this study, bacteria isolated from traditional dairy products in Guilan province. Exopolysaccharides extraction were performed and the presence of their producing genes was confirmed. Also, their antioxidant and anticancer properties were evaluated on breast cancer cell line (MCF7) using MTT assay. In this study, 7 bacteria isolated from traditional dairy products of Guilan province that had probiotic properties and were capable of producing exopolysaccharides were screened and phenotypic and genotypic identification were performed. Also, antioxidant and antitumor properties were evaluated by the free radical assay method of 1 and 1-dimethyl-2-pyridylhydrazine and lactate dehydrogenase enzyme, respectively, and their anticancer effect on breast cancer cell line (MCF7) was evaluated by the MTT test. The results indicated that all seven isolates had probiotic properties. The best isolate with the highest exopolysaccharide production in molecular identification was Lacnobacillus plantarum. The presence of exopolysaccharide-producing genes in the isolates was. The highest toxicity against the MCF7 breast cancer cell line by the best sample IC50 was 7.83 mg/L. Also, the extracted exopolysaccharide of the best isolate showed strong antioxidant and antitumor properties. The use of extracted exopolysaccharide after confirmation and supplementary tests in the inventory is recommended for therapeutic uses.
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| Keywords: Lactobacillus, exopolysaccharide, antioxidant, antitumor |
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Type of Study: Research |
Subject:
General
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