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Isolation and identification of Lactobacillus species isolated from the vagina of healthy women and phenotypic and genotypic study of their antibiotic resistance
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Masoumeh Anvari *   , Azadeh Eftekhari , Meysam Hasannejad-Bibalan |
| Department of Biology, Faculty of Basic Sciences, Rasht Branch, Islamic Azad University, Rasht |
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Abstract: |
One of the important factors in the use of probiotics is the isolation and identification of new strains without drug resistance with probiotic potential. The aim of this study was to isolate and identify bacteria belonging to the genus Lactobacillus and evaluate their drug resistance from healthy women referred to the Mehr infertility treatment clinic of Rasht. 50 samples were prepared and after that they were cultured on MRS agar. Also, morphological and biochemical identification of the isolates was performed. Sugar fermentation, hemolysis, acid tolerance and resistance to bile salts tests were performed. Resistance to common antibiotics was examined phenotypically by the disk diffusion method and MIC determination and the presence of resistance genes in the isolated lactobacilli were genetically assayed. Finally, the most sensitive strains with probiotic properties were molecularly identified using the 16SrRNA gene PCR reaction. 12 isolates had grown better and were observed as gram-positive bacilli in Gram stain. Phenotypic identification confirmed the genus Lactobacillus. Sugar fermentation test was positive and hemolysis and catalase test was negative for all of isolates. The bacteria were also able to grow in acidic pH and bile salts. The best isolate in phenotypic methods and molecular analysis lacked acquired drug resistance genes was identified as Lactobacillus crispatus. By conducting further complementary Invivo tests on the probiotic properties of this microorganism and its health-promoting effects, it can be used as a therapeutic supplement for medicinal purposes such as vaginal suppositories.
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| Keywords: Probiotic properties, Lactobacillus, Drug resistance, Vagina |
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Type of Study: Research |
Subject:
General
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