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Study on the isolation and characterization of new bacterial strains producing active substances at the biosurfactant level from municipal waste in Avuj city
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Nazanin zahra Eftekhar   , Behzad Hemati * |
| Department of Microbiology, Faculty of Basic Sciences, Ka.C., Islamic Azad University, Karaj, Iran |
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Abstract: |
| Biosurfactants are surface-active substances produced by microorganisms that reduce surface and interfacial tension. In recent years, increasing environmental awareness has attracted much attention towards the use of biosurfactants compared to their chemical counterparts, which is due to the unique properties of these compounds. Isolation of new strains with high biosurfactant production capacity has always been important for researchers. This study aimed to isolate and characterize a new and indigenous thermophilic isolate with high biosurfactant production capacity from municipal waste and to investigate its physicochemical properties. Among more than 120 colonies isolated from different sources, one isolate with a biosurfactant production rate of more than 12 g/L was selected and identified by biochemical and molecular morphological methods based on the S 16 rRNA gene. Initial identification of the biosurfactant was performed by FT-IR. Investigation of the antimicrobial properties of the purified biosurfactant showed that among the pathogenic bacteria studied in this study, Staphylococcus aureus and Klebsiella pneumoniae strains were more sensitive to it and it could also maintain its surface activity in a wide range of pH (3-9), high salt concentrations (up to 18%), and boiling temperatures of autoclaves, freezers, and refrigerators. The critical micellization concentration of the biosurfactant was 39 mg/L, at which point the surface tension was 40.5 mN/m. |
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| Keywords: Biosurfactant, municipal waste, new bacterial strain, isolation, antimicrobial. |
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Type of Study: Research |
Subject:
General
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