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Molecular monitoring of contamination of water resorts in Mazandaran province with some adenovirus strains
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Fatemeh Jamalifard   , فاطمه Roodbari * , Salman Ahmady asbchin |
| Department of Pathogenic Microbiology, University of Mazandaran, Babolsar, Iran |
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Abstract: |
Adenoviruses are associated with various diseases such as respiratory infections and eye infections. A variety of biological contaminants can reduce water quality. Adenoviruses are considered a suitable model for assessing the pollution of aquatic environments due to their environmental resistance and abundance throughout the year. Therefore, this study investigated adenoviruses and their relationship with water pollution. For this purpose, 60 samples were collected from recreational water areas. The temperature and pH of the water samples were also measured and filtered. Genome extraction was performed according to the kit instructions. Primers for types 3, 7, 40, and 41 were designed and examined in the BLAST program. PCR was used to identify the viruses. In this study, using general primers, 9 samples were positive, but specific serotypes were not identified. There was no statistically significant difference between pH and the presence of the virus, but there was a significant relationship between low temperatures and the prevalence of the virus. Although specific serotypes were not identified in this study, the presence of adenovirus in the waters studied was confirmed. On the other hand, the presence of adenovirus in pools containing clean water highlights the need for greater monitoring by authorities.
Keywords: Human adenovirus, recreational waters, PCR, filtration
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| Keywords: Human adenovirus, recreational waters, PCR, filtration |
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Type of Study: Research |
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