Comparative Investigation of The Antibacterial Properties of Silver Nanoparticles Biosynthesized by The Green Skin of Walnut Against Streptococcus Mutans and Pseudomonas Aeruginosa
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Roya Parasa , Younes Anzabi * |
Associate Professor, Department of Pathobiology, Faculty of Veterinary Medicine, Tabriz Medical Sciences, Islamic Azad University, Tabriz, Iran. |
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Abstract: |
Due to the indiscriminate use of disinfectants all over the world in order to disinfect the environment, which has led to the creation of cross-resistance between different antimicrobial substances and new, resistant strains and the production of superviruses and resistant bacteria, in recent years, The main focus of medical science research has been on nanotechnology. Therefore, according to these two issues, in the present study, an attempt has been made to prepare a nano disinfectant based on plant metabolites so that it is safe, durable and can replace alcohol. Therefore, the inhibition of 2 bacteria Streptococcus mutans ATCC35668 and Pseudomonas aeruginosa PTCC1310 by aqueous and ethanolic extracts and The biosynthesized nanoparticle was investigated by the alcoholic extract of green walnut skin. to check the antibacterial properties of the materials, MIC (Minimum Inhibitory Concentration), MBC (Minimum Bactericidal Concentration) of the materials were measured. MIC levels of aqueous, ethanolic extracts and biosynthesized nanoparticles of green walnut skin for both Streptococcus mutans and Pseudomonas aeruginosa bacteria were 1.4, 0.7 and 0.1, respectively, and MBC was 2.8, 1.4 and 0.1 milliliters. It is estimated in grams per milliliter. Also, the MIC and MBC of 96% alcohol were estimated between 0.7 and 2.8 mg/ml, which indicated the strong biosynthesis ability of green walnut skin and its strong antibacterial properties. It seems that silver nanoparticles biosynthesized by green walnut skin can be used as a suitable disinfectant instead of alcohol and its similar compounds against Gram-positive and Gram-negative bacteria.
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Keywords: Silver Nanoparticle, Alcohol, Streptococcus Mutans Bacteria, Pseudomonas Aeruginosa, Green Walnut Skin Extract, Silver Nanoparticle, Alcohol, Streptococcus Mutans Bacteria, Pseudomonas Aeruginosa |
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Type of Study: Research |
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