Applied Microbiology in Food Industry

fa Impact of Native Iranian Probiotic Mixture on the expression of Tau protein, Memory ‎Impairment and Antioxidant Markers in a Beta-Amyloid-Induced ‎Alzheimer's Rat Model Impact of Native Iranian Probiotic Mixture on the expression of Tau protein, Memory ‎Impairment and Antioxidant Markers in a Beta-Amyloid-Induced ‎Alzheimer's Rat Model میکروبیولوژی مواد غذایی General مروری Review <div dir="ltr">Background and Aim : Alzheimer's disease, a prevalent neurodegenerative disorder affecting the elderly, is &lrm;characterized by the accumulation of amyloid-beta and tau proteins. Recent research suggests &lrm;that a well-balanced microbiome, modulated by probiotics, may alleviate AD symptoms by &lrm;enhancing antioxidant production and neutralizing reactive oxygen species in the &lrm;gastrointestinal tract. This study examined the effects of a probiotic mixture containing &lrm;Lactobacillus acidophilus, L. paracasei, L. rhamnosus, L. reuteri, L. coagulans, and &lrm;Bifidobacterium longum on memory impairment and antioxidant levels in a rat model of Alzheimer's induced by beta-amyloid injection&lrm; Methods : Twenty-one rats were divided into three groups: control group, and two experimental groups in &lrm;which Alzheimer's disease was induced by beta-amyloid injection into the CA1 region. One &lrm;experimental group received probiotics for three weeks, while the other was given distilled &lrm;water. The study involved conducting behavioral assessments such as the shuttle box and &lrm;elevated plus maze tests. Additionally, ELISA analyses levels of antioxidant markers, and western blot &lrm;was used to assess the Tau protein expression&lrm; Results : The results showed that beta-amyloid injection significantly decreased cognitive function, increased tau protein expression and MDA serum level, and decreased SOD activity. However, probiotic treatment significantly improved learning, reduced oxidative stress, and enhanced neuronal regeneration. Conclusion : These findings suggest that indigenous Iranian probiotics can enhance cognitive function and &lrm;mitigate AD symptoms through their antioxidant effects&lrm;.  </div> Background and Aim : Alzheimer's disease, a prevalent neurodegenerative disorder affecting the elderly, is &lrm;characterized by the accumulation of amyloid-beta and tau proteins. Recent research suggests &lrm;that a well-balanced microbiome, modulated by probiotics, may alleviate AD symptoms by &lrm;enhancing antioxidant production and neutralizing reactive oxygen species in the &lrm;gastrointestinal tract. This study examined the effects of a probiotic mixture containing &lrm;Lactobacillus acidophilus, L. paracasei, L. rhamnosus, L. reuteri, L. coagulans, and &lrm;Bifidobacterium longum on memory impairment and antioxidant levels in a rat model of Alzheimer's induced by beta-amyloid injection&lrm; Methods : Twenty-one rats were divided into three groups: control group, and two experimental groups in &lrm;which Alzheimer's disease was induced by beta-amyloid injection into the CA1 region. One &lrm;experimental group received probiotics for three weeks, while the other was given distilled &lrm;water. The study involved conducting behavioral assessments such as the shuttle box and &lrm;elevated plus maze tests. Additionally, ELISA analyses levels of antioxidant markers, and western blot &lrm;was used to assess the Tau protein expression&lrm; Results : The results showed that beta-amyloid injection significantly decreased cognitive function, increased tau protein expression and MDA serum level, and decreased SOD activity. However, probiotic treatment significantly improved learning, reduced oxidative stress, and enhanced neuronal regeneration. Conclusion : These findings suggest that indigenous Iranian probiotics can enhance cognitive function and &lrm;mitigate AD symptoms through their antioxidant effects&lrm;.   1 1 http://amfi.ir/browse.php?a_code=A-10-1-8&slc_lang=fa&sid=1 Zohreh Shadmehr 10031947532846001199 10031947532846001199 Yes Department of Biology, Science and Research Unit, Islamic Azad University Bahareh Pakpuor 10031947532846001200 10031947532846001200 No Department of biology, Central Tehran Branch, Islamic Azad University, ‎Tehran, Iran Navaeian3 Navaeian 10031947532846001201 10031947532846001201 No Department of Biology, Yadegar-e-Imam Khomeini (RAH) Shahre Rey ‎Branch, Islamic Azad University, Tehran, Iran‎ Maryam Taj Abadi Ebrahimi 10031947532846001202 10031947532846001202 No