fa جداسازی و شناسایی سویه بومی باسیلوس مولد آلفا آمیلاز گرمادوست از منابع آب گرم شهرستان آوج میکروبیولوژی مواد غذایی General پژوهشي Research <span style="font-size:12px;"><span style="line-height:99%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><span lang="FA"><span style="background:yellow"><span style="line-height:99%"><span b="" zar=""><span style="background-color:#ffffff;">مقدمه:آﻟﻔﺎآﻣﻴﻼزﻫﺎ، ﻣﻬﻢﺗﺮﻳﻦ و ﭘﺮﻛﺎرﺑﺮدﺗﺮﻳﻦ آﻣﻴﻼزﻫﺎ در ﺻﻨﻌﺖ ﻣﻲﺑﺎﺷﻨﺪ. در ﻣﻴﺎن آﻟﻔﺎآﻣﻴﻼزﻫﺎ، اﻧﻮاع ﮔﺮﻣﺎدوﺳﺖ ﺑﻪ دﻟﻴﻞ ﭘﺎﻳﺪاری و ﻓﻌﺎﻟﻴﺖ در دﻣﺎﻫﺎی ﺑﺎﻻ از اﻫﻤﻴﺖ وﻳﮋه&shy;ای ﺑﺮﺧﻮردارﻧﺪمواد و روش&shy;ها: در اﻳﻦ ﺗﺤﻘﻴﻖ، به ترتیب ابتدا نمونه برداری، جداسازی و غربالگری سویه های باکتریایی مولد آلفاآمیلاز، شناسایی سویۀ باکتریایی جداشده، سنجش فعالیت آنزیم، سنجش فعالیت آلفاآمیلاز، اثر منابع مختلف کربن بر تولید آنزیم آلفاآمیلاز، بررسی اثر</span></span></span></span></span><span dir="LTR"><span style="background:yellow"><span style="line-height:99%"><span new="" roman="" times=""><span style="background-color:#ffffff;">pH</span></span></span></span></span><span style="background-color:#ffffff;"> </span><span lang="FA"><span style="background:yellow"><span style="line-height:99%"><span b="" zar=""><span style="background-color:#ffffff;">بر تولید آنزیم آلفاآمیلاز، بررسی فعالیت دمایی آنزیم آلفاآمیلاز، &nbsp;بررسی پایداری دمایی آنزیم آلفاآمیلاز انجام شد.نتایج: ﺑﺮ اﺳﺎس ﻧﺘﺎﻳﺞ ﻏﺮﺑﺎﻟﮕﺮی در ﻣﺤﻴﻂ اﺧﺘﺼﺎﺻﻲ ﻣﺎﻳﻊ و ﺟﺎﻣﺪ، دو ﺳﻮﻳﻪ ﺑﺎ ﻛﺪﻫﺎی </span></span></span></span></span><span dir="LTR"><span style="background:yellow"><span style="line-height:99%"><span new="" roman="" times=""><span style="background-color:#ffffff;">TSG59</span></span></span></span></span><span lang="FA"><span style="background:yellow"><span style="line-height:99%"><span b="" zar=""><span style="background-color:#ffffff;"> و </span></span></span></span></span><span dir="LTR"><span style="background:yellow"><span style="line-height:99%"><span new="" roman="" times=""><span style="background-color:#ffffff;">TSG70</span></span></span></span></span><span lang="FA"><span style="background:yellow"><span style="line-height:99%"><span b="" zar=""><span style="background-color:#ffffff;">، ﺑﻪ ﻋﻨﻮان ﺳﻮﻳﻪﻫﺎی ﺑﺮﺗﺮ اﻧﺘﺨﺎب ﮔﺮدﻳﺪﻧﺪ. ﺷﻨﺎﺳﺎﻳﻲ ﺑﻴﻮﺷﻴﻤﻴﺎﻳﻲ&shy;&shy;</span></span></span></span></span><span lang="FA"><span style="background:yellow"><span style="line-height:99%"><span cambria=""><span style="background-color:#ffffff;">_</span></span></span></span></span><span lang="FA"><span style="background:yellow"><span style="line-height:99%"><span b="" zar=""><span style="background-color:#ffffff;">ﻣﻮﻟﻜﻮﻟﻲ ﻧﺸﺎن داد </span></span></span></span></span><span dir="LTR"><span style="background:yellow"><span style="line-height:99%"><span new="" roman="" times=""><span style="background-color:#ffffff;">TSG59</span></span></span></span></span><span style="background:yellow"><span style="line-height:99%"><span b="" zar=""><span style="background-color:#ffffff;"> و </span></span></span></span><span dir="LTR"><span style="background:yellow"><span style="line-height:99%"><span new="" roman="" times=""><span style="background-color:#ffffff;">TSG70</span></span></span></span></span><span lang="FA"><span style="background:yellow"><span style="line-height:99%"><span b="" zar=""><span style="background-color:#ffffff;"> ﺑا </span></span></span></span></span><i><span dir="LTR"><span style="background:yellow"><span style="line-height:99%"><span new="" roman="" times=""><span style="background-color:#ffffff;">Bacillus licheniformis</span></span></span></span></span></i><span lang="FA"><span style="background:yellow"><span style="line-height:99%"><span b="" zar=""><span style="background-color:#ffffff;"> ﺑﻴﺸﺘﺮﻳﻦ ﺷﺒﺎﻫﺖ را داﺷﺘﻪاﻧﺪ. ﻧﺘﺎﻳﺞ ﺑﻬﻴﻨﻪﺳﺎزی ﻣﺤﻴﻂ ﺗﻮﻟﻴﺪ آﻧﺰﻳﻢ ﻧﺸﺎن داد ﺗﻮﻟﻴﺪ آﻟﻔﺎآﻣﻴﻼز دو ﺳﻮﻳﻪ </span></span></span></span></span><span dir="LTR"><span style="background:yellow"><span style="line-height:99%"><span new="" roman="" times=""><span style="background-color:#ffffff;">TSG59</span></span></span></span></span><span style="background-color:#ffffff;"> </span><span lang="FA"><span style="background:yellow"><span style="line-height:99%"><span b="" zar=""><span style="background-color:#ffffff;">و </span></span></span></span></span><span dir="LTR"><span style="background:yellow"><span style="line-height:99%"><span new="" roman="" times=""><span style="background-color:#ffffff;">TSG70</span></span></span></span></span><span style="background-color:#ffffff;"> </span><span lang="FA"><span style="background:yellow"><span style="line-height:99%"><span b="" zar=""><span style="background-color:#ffffff;">ﺑﻪ ﺗﺮﺗﻴﺐ در ﻣﺤﻴﻂ ﺣﺎوی ٥ ﮔﺮم ﺑﺮ ﻟﻴﺘﺮ ﻧﺸﺎﺳﺘﻪ، ٢ ﮔﺮم ﺑﺮ ﻟﻴﺘﺮ ﭘﭙﺘﻮن، ﻳﻚ ﮔﺮم ﺑﺮ ﻟﻴﺘﺮ ﻛﻠﺴﻴﻢ ﻛﻠﺮﻳﺪ و 5 </span></span></span></span></span><span dir="LTR"><span style="background:yellow"><span style="line-height:99%"><span new="" roman="" times=""><span style="background-color:#ffffff;">pH</span></span></span></span></span><span style="background-color:#ffffff;"> </span><span lang="FA"><span style="background:yellow"><span style="line-height:99%"><span b="" zar=""><span style="background-color:#ffffff;">و ﻣﺤﻴﻂ ﺣﺎوی ٥ ﮔﺮم ﺑﺮ ﻟﻴﺘﺮ ﻧﺸﺎﺳﺘﻪ، ٢ ﮔﺮم ﺑﺮ ﻟﻴﺘﺮ آﻣﻮﻧﻴﻮم ﻛﻠﺮاﻳﺪ، ﻳﻚ ﮔﺮم ﺑﺮ ﻟﻴﺘﺮ ﻛﻠﺴﻴﻢ ﻛﻠﺮﻳﺪ و 8 </span></span></span></span></span><span dir="LTR"><span style="background:yellow"><span style="line-height:99%"><span new="" roman="" times=""><span style="background-color:#ffffff;">pH</span></span></span></span></span><span style="background-color:#ffffff;"> </span><span lang="FA"><span style="background:yellow"><span style="line-height:99%"><span b="" zar=""><span style="background-color:#ffffff;">ﺑﻴﺸﺘﺮﻳﻦ ﻣﻘﺪار ﺑﻮده اﺳﺖ.ﺣﺪاﻛﺜﺮ ﻓﻌﺎﻟﻴﺖ و ﭘﺎﻳﺪاری آﻟﻔﺎآﻣﻴﻼز </span></span></span></span></span><span dir="LTR"><span style="background:yellow"><span style="line-height:99%"><span new="" roman="" times=""><span style="background-color:#ffffff;">TSG59</span></span></span></span></span><span style="background-color:#ffffff;"> </span><span lang="FA"><span style="background:yellow"><span style="line-height:99%"><span b="" zar=""><span style="background-color:#ffffff;">در دﻣﺎ 50&nbsp; درﺟﻪ سلسیوس ﻣﺸﺎﻫﺪه ﺷﺪ و ﺑﺮای آﻟﻔﺎآﻣﻴﻼز </span></span></span></span></span><span dir="LTR"><span style="background:yellow"><span style="line-height:99%"><span new="" roman="" times=""><span style="background-color:#ffffff;">TSG70</span></span></span></span></span><span lang="FA"><span style="background:yellow"><span style="line-height:99%"><span b="" zar=""><span style="background-color:#ffffff;">، ﺑﻪ ﺗﺮﺗﻴﺐ در ٦٠ و ٧٠ درﺟﻪ سلسیوس ﺑﻪ دﺳﺖ آﻣﺪ، ﭘﺎﻳﺪاری دﻣﺎﻳﻲ ﻫﺮ دو </span></span></span></span></span><span dir="LTR"><span style="background:yellow"><span style="line-height:99%"><span new="" roman="" times=""><span style="background-color:#ffffff;">TSG59</span></span></span></span></span><span style="background-color:#ffffff;"> </span><span lang="FA"><span style="background:yellow"><span style="line-height:99%"><span b="" zar=""><span style="background-color:#ffffff;">و </span></span></span></span></span><span dir="LTR"><span style="background:yellow"><span style="line-height:99%"><span new="" roman="" times=""><span style="background-color:#ffffff;">TSG70</span></span></span></span></span><span style="background-color:#ffffff;"> </span><span lang="FA"><span style="background:yellow"><span style="line-height:99%"><span b="" zar=""><span style="background-color:#ffffff;">ﺑﺎ اﺿﺎﻓﻪ ﻛﺮدن ﻛﻠﺴﻴﻢ ﻛﻠﺮﻳﺪ ٢٠ ﻣﻴﻠﻲﻣﻮﻻر ﺑﻬﺒﻮد ﭘﻴﺪا ﻛﺮد.</span></span></span></span></span></span></span></span></span></span> <span style="font-size:11pt"><span style="line-height:150%"><span style="font-family:Calibri,sans-serif"><span style="font-size:10.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times="">Introduction: Alpha-amylases are the most important and widely used amylases in industry. Among alpha-amylases, thermophilic strains are of particular importance due to their stability and activity at high temperatures. Materials and Methods: In this study, sampling, isolation, and screening of alpha-amylase-producing bacterial strains, identification of the isolated bacterial strain, measurement of enzyme activity, measurement of alpha-amylase activity, effect of different carbon sources on alpha-amylase enzyme production, investigation of the effect of pH on alpha-amylase enzyme production, investigation of alpha-amylase enzyme thermal activity, investigation of alpha-amylase enzyme thermal stability were performed, respectively. Results: Based on the results of screening in special liquid and solid media, two strains with codes TSG59 and TSG70 were selected as superior strains. Molecular biochemical identification showed that TSG59 and TSG70 had the highest similarity to Bacillus licheniformis. The results of the optimization of the enzyme production medium showed that the production of bi-strain alpha-amylase TSG59 and TSG70 was highest in a medium containing 5 g/L starch, 2 g/L peptone, 1 g/L calcium chloride and pH 5, respectively, and in a medium containing 5 g/L starch, 2 g/L ammonium chloride, 1 g/L calcium chloride and pH 8. The maximum activity and stability of alpha-amylase TSG59 was observed at a temperature of 50 degrees Celsius, and for alpha-amylase TSG70, respectively, 60 and 70 degrees Celsius were achieved, the thermal stability of both TSG59 and TSG70 was improved by adding 20 mM calcium chloride. </span></span></span></span></span></span><br> &nbsp; آلفا آمیلاز,تولید,آنزیم‌های گرما دوست,فعالیت دمایی,پایداری دمایی Alpha-amylase,Production,Friend heat enzymes,Temperature activity,Temperature stability. 46 69 http://amfi.ir/browse.php?a_code=A-10-205-1&slc_lang=fa&sid=1 Nazanin zahra Eftekhar نازنین زهرا افتخار mahnaz.aab@gmail.com 10031947532846001097 10031947532846001097 No - Behzad Hemati بهزاد همتی hematibehzad71@gmail.com 10031947532846001098 10031947532846001098 Yes Member of the academic faculty of Azad University, Karaj branch, rank 17 عضو هیئت علمی دانشگاه آزاد واحد کرج-دانشیار رتبه 17